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KMID : 1094719970020010019
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Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 1 p.19 ~ p.19
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Production of maltooligosaccharides from starch and separation of maltopentaose by adsorption of them on activated carbon (I)
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Moon Il-Shik
Cho Gyou-Jin
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Abstract
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For the selective production of maltopentaose (G5) over other oligosaccharides, enzymatic hydrolysis conditions of starch by commercial α-amylase (Termamyl®) were investigated. The determined optimum condition was 29.6 KNU (Kilo Novo α-amylase Unit) enzyme loading in 150 mL of 0.3% starch solution under pH level of 5 at 40°C for 30 min. About 40% of G5 selectivity can be attained using the determined optimum condition. For further enhancing G5 selectivity, an activated carbon adsorption process has been attached after the enzymatic hydrolysis. From the adsorption process, G5 can be enriched up to 72% in the solution.
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KEYWORD
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maltooligosaccharides, maltopentaose, α-amylase, starch, activated carbon
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